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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in many tumors, which leads to chromosome instability, and so could lead to tumorigenesis. This protein was located to affiliate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a critical position in endorsing cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two more splice variants were being noted but detailed nucleotide sequence information and facts is not really nevertheless available.
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Blocking peptides are peptides that bind especially for the concentrate on antibody and block antibody binding. These peptide typically consists of the epitope recognized via the antibody.
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This manual outlines further possible optimization of mobile and immunoassay parameters to be sure the absolute best final results are...
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This guide outlines additional feasible optimization of cellular and immunoassay parameters to make certain the absolute best benefits are...
Antibodies certain to the blocking peptide not bind on the epitope about the focus on protein. By comparing the staining from your blocked antibody compared to the antibody alone, you can see which staining is specific.
Focus on Information Cyclin E belongs to the highly conserved cyclin spouse and children, whose users are characterised by a remarkable periodicity in protein abundance with the cell cycle. Cyclins operate as regulators of CDK kinases. Distinctive cyclins exhibit distinct expression and degradation designs which lead towards the temporal coordination of each and every mitotic party. Cyclin E varieties a complex with and capabilities for a regulatory subunit of CDK2, whose activity is necessary for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and is also degraded as cells development by S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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